Monoacetylation of 2,4-Diamino-6-Nitrotoluene. Kinetic Studies with α-Chymotrypsin. Effect of Dilantin Upon Ether Narcosis in the Cat

Author: Foster, Robert Joseph

Year: 1952

Degree: Dissertation (Ph.D.)

Advisor: Niemann, Carl G.

Committee Member: Unknown, Unknown

Option: Chemistry; Biology

DOI: 10.7907/K13Z-9D57

Abstract

The structure of the monoacetyl derivative of 2,4-diamino-6-nitrotoluene formed by reaction with an equimolar quantity of acetic anhydride in dry acetone at room temperature has been shown to be 2-amino-4-acetamido-6-nitrotoluene.

The kinetic constants for the enantiomorphic pair of acetyl-phecylalaninamides and α-chymotrypsin at 25°and pH 7.9 in aqueous solution have been determined. The relationships of these constants with others determined for similar enantiomorphic pairs is discussed. Equations for the competitive hydrol­ysis of two substrates at the same catalytically active site of an enzyme have been derived and have been tested experimentally.

The kinetic constants for L-tyrosine-hydroxamide and α-chymotrypsin have been determined at pH 6.9 and 25°. With this hydroxamide as substrate, the enzyme-inhibitor complex dis­sociation constants have been determined for a series of inhibitors at pH 6.9 and 25°. The differences between these constants and those at pH 7.9 is discussed and the charge state of the catalytically active site is inferred.

The effect of the anti-convulsant dilantin upon ether narcosis in the cat has been determined and is discussed in its relation to present theories of nervous propagation and narcosis.

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