Developing Technologies for Real-Time Whole-Organism Imaging via FTIR Spectromicroscopy

Author: Holman, Elizabeth Anne

Year: 2022

Degree: Dissertation (Ph.D.)

Advisors: Sternberg, Paul W.; Rees, Douglas C.

Committee Members: Davis, Mark E.; Grubbs, Robert H.; Okumura, Mitchio; Sternberg, Paul W.; Rees, Douglas C.

Option: Chemistry

DOI: 10.7907/es4r-kq84

Abstract

FTIR spectromicroscopy heavily resides in the domain of cell-based and tissue-based studies when focusing on its direct application to biological systems. The goal of the reported graduate research is to extend FTIR spectromicroscopy to multicellular whole-organism imaging, ideally for non-invasive, non-destructive, and label-free spatiochemical imaging of biological model Caenorhabditis elegans (C. elegans). With modern optics, detector, and light source technologies implemented at synchrotron facilities, this thesis focuses on exploring the feasibility of multicellular whole-organism imaging while identifying challenges and presenting working solutions for them.

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