Electron Transfer in Structurally Engineered Metalloproteins

Author: Mayo, Stephen Leon

Year: 1988

Degree: Dissertation (Ph.D.)

Advisors: Campbell, Judith L.; Gray, Harry B.; Richards, John H.

Committee Members: Goddard, William A., III; Gray, Harry B.; Campbell, Judith L.; Richards, John H.; Dervan, Peter B.; Simon, Melvin I.

Option: Chemistry

DOI: 10.7907/YJKW-3133

Abstract

This thesis is concerned with experimental approaches for studying electron transfer in metalloproteins. The material presented traces the development of experimental techniques aimed at elucidating the mechanism of intramolecular metalloprotein electron transfer. The first chapter includes a reprint of an Inorganic Chemistry paper that describes the bimolecular reaction of small inorganic redox complexes with blue-copper proteins. The second chapter illustrates the use of covalently attached redox probes for studying rate-distance relationships. And the third chapter documents our progress in using genetic methods to structurally engineer yeast iso-1 cytochrome c into an idealized system for studying the distance dependence of intramolecular electron-transfer events.

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