Preparation and Characterization by Streaming Birefringence of Sodium Desoxyribonucleate

Author: Hershman, Jerome Marshall

Year: 1954

Degree: Master's thesis

Advisor: Rich, Alexander

Committee Member: Unknown, Unknown

Option: Chemistry

DOI: 10.7907/8XHJ-5E14

Abstract

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Three preparations of the sodium salt of desoxyribonucleic acid were isolated from calf thymus by a method expected to give minimal or no depolymerization. In addition, preparations were made from calf spleen and bull testis tissues.

Streaming birefringence measurements indicate that the distributions of molecular length are identical in the calf thymus preparations. The apparent molecular lengths range from 7,500 to 11,500[...]. The sodium desoxyribonucleate from testis appeared to be significantly shorter; that from spleen was intermediate between thymus and testis. The distributions of molecular length of two calf thymus preparations isolated by N. Simmons were compared with those prepared here.

Several explanations are put forth to explain the polydispersity and discrepancies between preparations.

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