The Fractionation of Serum Proteins by Electrophoresis-Convection

Author: Brown, Raymond Allen

Year: 1949

Degree: Dissertation (Ph.D.)

Advisor: Unknown, Unknown

Committee Member: Unknown, Unknown

Option: Chemistry; Physics

DOI: 10.7907/43QN-WP04

Abstract

NOTE: Text or symbols not renderable in plain ASCII are indicated by [...]. Abstract is included in .pdf document.

A new improved electrophoresis-convection apparatus for the fractionation of proteins is described. The potentialities of the apparatus are demonstrated in the partial separation of bovine serum proteins and in the sub-fractionation of bovine [...]-globulin, Fraction II obtained by alcohol-salt precipitation. A fraction containing 96% [...]-globulin has been isolated from bovine serum. Bovine [...]-globulin has been separated into eight fractions with mobilities ranging from -1.25x10(-5) to -2.25x10(-5) and isoelectric points extending from pH 7.31 to pH 5.74.

An extension of the theory of reversible boundary spreading in the electrophoresis of proteins is presented. The theory has been applied to the characterization of the [...]-globulin fractions.

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