In Vitro Selection of mRNA-Display Libraries Containing Unnatural Amino Acids

Author: Li, Shuwei

Year: 2003

Degree: Dissertation (Ph.D.)

Advisor: Roberts, Richard W.

Committee Members: Dervan, Peter B.; Bercaw, John E.; Roberts, Richard W.; Mayo, Stephen L.

Option: Chemistry

DOI: 10.7907/NVCQ-JS95

Abstract

The mRNA display library provides a strategy to make up to 10¹³ unique peptides that fuse with their own genes via a covalent link. In this thesis, several strategies to prepare mRNA display libraries containing unnatural side chains are described. Chapter 1 is a short introduction and application of mRNA-display technology. Chapter 2 describes a chemical derivatization strategy to append an unnatural side chain to libraries. Chapter 2 introduces the incorporation of unnatural amino acids via non-sense codon suppression. Chapter 4 focuses on the catalytic selection with mRNA display libraries. These libraries should facilitate the discovery of novel ligands with functionalities beyond those provided by the 20 naturally occurring amino acids.

Files

• Acknowledgements.pdf (application/pdf)
• allfile.pdf (application/pdf)
• Chapte1.pdf (application/pdf)
• chapter2.pdf (application/pdf)
• Chapter3.pdf (application/pdf)
• Chapter4.pdf (application/pdf)