Nucleic Acid Interactions II. Chemical Linkage of the Carcinogen 3,4-Benzpyrene to DNA Induced by Photoradiation

Authors: Lu, Ponzy; Ts'o, Paul On Pong

Year: 1964

Degree: Bachelor's thesis

Advisor: Bonner, James Frederick

Committee Member: Unknown, Unknown

Option: Chemistry

DOI: 10.7907/dr6e-hb15

Abstract

The physical binding of benzpyrene-H³ with coil form of thymus DNA has been studied. Covalent linkage between the benzpyrene-H³ or the degradation products of benzpyrenc-H³ and DNA was generated by photoradiation at the absorption band of benzpyrene with the "yield" of photoproduct around 50%. This finding has been conclusively supported by experiments with precipitation and extraction, sedimentation, electrophoresis, spectroscopy, enzymic hydrolysis, and chromatography of the hydrolytic products.

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