Electron Microscopy of DNA

Author: Eyler, John Robert

Year: 1967

Degree: Bachelor's thesis

Advisor: Davidson, Norman R.

Committee Member: Unknown, Unknown

Option: Chemistry

DOI: 10.7907/3pfb-8987

Abstract

Studies of the pre-formed film technique, used to prepare DNA for electron-microscopic investigation gave results of generally poor reliability, but some data and analyses contradict a conclusion that the average DNA molecule projects its spatial conformation onto a plane during adsorption at the protein-water interface in the pre-formed film technique, which was presented in a paper by Lang, Bujard, et.al.

Electron-microscopic investigation of single-stranded regions of λ DNA produced by reaction with methylmercuric hydroxide (CH₃HgOH) gave promising results which indicate this method may be an effective one for locating and examining A-T rich regions in DNA.

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