Low Molecular Weight Substrates for Lysozyme

Author: Brown, Larry Robert

Year: 1968

Degree: Bachelor's thesis

Advisor: Raftery, Michael A.

Committee Member: Unknown, Unknown

Option: Chemistry

DOI: 10.7907/fsaw-ew37

Abstract

Preparation of short chain polymers of N-acetylglucosamine by acid hydrolysis of chitin was undertaken. Separation of the various chain lengths of the hydrolysis breakdown products was done by column chromatography on charcoal-celite with an ethanol solvent.

Preparation of the dimer and tetramer of N-acetylglucosaineN- acetylmuramic acid was undertaken by lysozyme digestion of the purified cell walls of Micrococcus lysodeikticus. Separation was again accomplished by chromatography.

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