I. Structural Analogs of Typical Substrates of Alpha-Chymotrypsin. II. l-Acetyl-2-[L-Tyrosyl] Hydrazine: an Inhibitor of Alpha-Chymotrypsin. Ill. Binuclear Aromatics as Inhibitors of Alpha-Chymotrypsin Catalyzed Hydrolyses. IV. Applicability of the pH-Stat to Alpha-Chymotrypsin Catalyzed Hydrolyses that Produce a Buffer

Author: Kurtz, Abraham Nathan

Year: 1960

Degree: Dissertation (Ph.D.)

Advisor: Niemann, Carl G.

Committee Member: Unknown, Unknown

Option: Chemistry

DOI: 10.7907/8C7N-4Z18

Abstract

Substitution of a nitrogen atom in place of the C-H group that occurs at the asymmetric center of typical substrates of alpha-chymotrypsin results in the complete loss of the ability of the enzyme to catalyze the hydrolysis of the carboethoxy or carboxamido group. However, the nitrogen-substituted analogs, which otherwise possess all of the remaining significant structural entities of typical substrates, function as inhibitors of alpha-chymotrypsin catalyzed hydrolyses but in general show less affinity for the enzyme than do the analogous C-H containing substrates.

It has been found that l-acetyl-2-[L-tyrosyl] hydrazine is a reversible inhibitor of alpha-chymotrypsin catalyzed hydrolyses with unusual affinity for the enzyme. At pH 7.9 the enzyme inhibitor constant was found to have a value of KI = 0.074 x 10(-3)M, which shows that this substance associates reversibly with alpha-chymotrypsin at the active site to a greater extent than any other known substrate or reversible inhibitor.

Preliminary results, based upon inhibition studies, indicate that alpha-chymotrypsin possesses a greater affinity for the naphthalene function than for the indole function.

A study of the applicability of the pH-stat to alpha-chymotrypsin catalyzed hydrolyses that produce a buffer showed that the reliability was a function of the buffer capacity. Amides gave poor results; hydrazides gave good or fair results, one of which contradicted a literature value, and a hydroxamide gave excellent results.

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