Part I. α-Secondary Isotope Effects on the Reaction Catalyzed by the Enzyme Fumarase. Part II. Salt Effects on the Enzyme Fumarase

Author: Schmidt, Donald Emil, Jr.

Year: 1967

Degree: Dissertation (Ph.D.)

Advisor: Richards, John H.

Committee Member: Unknown, Unknown

Option: Chemistry

DOI: 10.7907/D85P-2N72

Abstract

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PART I

The [alpha]-secondary isotope effect of L-malic-2-T-1, 4-C[superscript 14] acid on the reaction catalyzed by fumarase was determined. Evidence was presented which indicated that the reaction proceeded by formation of a carbonium ion in a rate-determining state at C-2 which possesses little sp[superscript 2] character.

PART II

The effects of sodium bromide and sodium phosphate at pH 7.3 and 27[degrees] were determined. A partial kinetic and mechanistic scheme has been developed to explain these effects.

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