An Ultracentrifugal Study of the Dissociation of Human Carboxyhemoglobin

Author: Hutchinson, William Day

Year: 1957

Degree: Master's thesis

Advisor: Vinograd, Jerome Rubin

Committee Member: Unknown, Unknown

Option: Chemistry

DOI: 10.7907/W8JC-7T49

Abstract

The dissociation of human carboxyhemoglobin has been investigated by means of sedimentation velocity measurements. Sedimentation studies on this protein have been extended to concentrations as low as 0.002% by the use of absorption optics. Hemoglobin was observed to dissociate increasingly as the pH was changed from 7 to 5. Apparent dissociation constants at each pH have been calculated. Values of the apparent specific volume of human carboxyhemoglobin have been found.

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