I. The Alpha-Chymotrypsin-Catalyzed and Base-Catalyzed Hydrolysis of Some Substituted Glycine Esters and Related Compounds. II. The Nature and Properties of the Enzyme-Substrate Complex Produced under Conditions of Very High Substrate Concentrations. III. A Hypothesis Concerning Alpha-Chymotrypsin-Substrate Complexes

Author: Wolf, John Peter

Year: 1959

Degree: Dissertation (Ph.D.)

Advisors: Niemann, Carl G.; Metcalf, Robert Lee

Committee Member: Unknown, Unknown

Option: Chemistry; Biology

DOI: 10.7907/KPJF-0G56

Abstract

A series of substituted glycogen esters have been evaluated as substrates for [alpha]-chymotrypsin. The second order base-catalyzed hydrolysis constants for these compounds have also been determined.

Four substrates for [alpha]-chymotrypsin have been found to exhibit unusual kinetic behavior at very high concentrations in the presence of [alpha]-chymotrypsin. It has been shown that these data can be the result of two molecules of substrate combining with each enzyme active site.

A hypothesis concerning the enzyme-substrate complex is offered which helps to correlate the data given in this work.

Files