Electrical Detection of DNA Binding Proteins

Author: Gorodetsky, Alon A.

Year: 2009

Degree: Dissertation (Ph.D.)

Advisor: Barton, Jacqueline K.

Committee Members: Heath, James R.; Gray, Harry B.; Barton, Jacqueline K.; Lewis, Nathan Saul

Option: Chemistry

DOI: 10.7907/VPKN-CV38

Abstract

The base pair stack of double helical DNA has proven to be an effective medium for charge transport. The π-stacked DNA base pairs can mediate charge transport (CT) chemistry over distances as long as 20 nm, and the reaction is exquisitely sensitive to DNA sequence-dependent conformation and dynamics. This sensitivity to perturbations in DNA structure and base pair stacking makes DNA-mediated charge transport chemistry an ideal methodology for the electrical detection of base mismatches, lesions, and protein binding. Efforts toward expanding the scope of electrochemistry at DNA-modified surfaces for biosensing applications are presented here.

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• Acknowledgements.pdf (application/pdf)
• Chapter1.pdf (application/pdf)
• Chapter10.pdf (application/pdf)
• Chapter2.pdf (application/pdf)
• Chapter3.pdf (application/pdf)
• Chapter4.pdf (application/pdf)
• Chapter5.pdf (application/pdf)
• Chapter6.pdf (application/pdf)
• Chapter7.pdf (application/pdf)
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• Chapter9.pdf (application/pdf)